abm | Human Primary Cardiomyocytes | T4037

The human cardiac (heart) muscle consists of unmarried cardiac myocytes. Cardiac myocytes are excessive strength cells accountable for wearing the maximum bodily lively cells within side the frame due to the fact they perform the myogenic contraction of the complete cardiac muscle. Promo Cell Human Cardiac Myocytes are generated from everyday human ventricle tissue of the person heart. Unlike freshly remoted rod-formed myocytes, Human Cardiac Myocytes are produced the use of a protocol that makes them amenable to long-time period experiments.

All Human Cardiac Myocytes are cryopreserved at passage 2 (P2) rapidly after isolation, the use of proprietary, serum-unfastened freezing medium, Cryo-SFM. Each thawed cryovial consists of as a minimum 500,000 feasible cells. Human Cardiac Myocytes also are to be had as proliferating mobileular cultures crafted from cryopreserved cells which have been thawed and cultured for 3 days in our labs.

Cultivation Protocol for Human Cardiac Myocytes

  • The Myocyte Growth Medium is optimized for developing number one human cardiac myocytes in vitro, however also can be used for number one porcine and rat myocytes.
  • The Myocyte Growth Medium is to be had in formats: Medium (ready-to-use) and Medium Kit.
  • The Medium (ready-to-use) alternative consists of a 500 mL bottle of Basal Medium and one vial of SupplementMix.
  • The Medium Kit consists of a 500 mL bottle of Basal Medium and the SupplementPack, that’s a fixed of character vials with pre-measured dietary supplements that offers the consumer complete manipulate over the very last media formulation.
  • Adding the SupplementMix or the SupplementPack to the Basal Medium constitutes the whole Myocyte Growth Medium.
  • The Basal Medium (without or with phenol red), SupplementMix, and SupplementPack also can be bought separately.

Cardiac Myocyte Growth Medium Supplementation Details

  • The Myocyte Growth Medium consists of all of the additives essential for the top-rated increase of human cardiac myocytes (see the desk under for details).
  • Myocyte Growth Medium does now no longer comprise antibiotics or antimycotics and is formulated to be used in an incubator with 5 % atmospheric CO2.
  • Preparing Supplemented Media for Cardiac Myocyte Culture Thaw the Myocyte Growth Medium SupplementMix or SupplementPack at 15 – 25 °C.
  • Aseptically blend the complement answers via way of means of pipetting up and down lightly.
  • Transfer the complete contents of every complement to the Myocyte Basal Medium.
  • Close the bottle and swirl lightly to shape a homogenous mixture.

Storage and Stability of Myocyte Products

Upon arrival, straight away shop the Myocyte Basal Medium withinside the darkish at 4 – 8 °C; do now no longer freeze. Store the Myocyte Growth Medium SupplementMix or SupplementPack at -20 °C. If saved as directed, the goods are strong till the expiration date said at the label. Once dietary supplements are brought to the Myocyte Basal Medium, the shelf lifestyles of the whole medium is six weeks at 4 – 8 °C. Do now no longer freeze the whole medium. To use, aliquot and prewarm simply the specified of the whole Myocyte Growth Medium and keep the last medium refrigerated at 4 – 8 °C. Upon arrival, shop cryopreserved cells in liquid nitrogen or seed directly. Proliferating cells need to be processed straight away.

QUALITY CONTROL FOR HUMAN CARDIAC MYOCYTES AND MYOCYTE GROWTH MEDIA

Human Cardiac Myocytes Quality Control The following inflexible best manipulate checks are done for every lot of Human Cardiac Myocytes: Cell morphology, adherence rate, and mobileular viability trying out Flow cytometry analyses for mobileular-kind particular markers consisting of sarcomeric alpha-actinin and gradual muscle myosin Growth overall performance trying out via a couple of passages up to fifteen populace doublings (PD) beneathneath tradition situations with out antibiotics and antimycotics Testing for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma) A specific certificates of analysis (CoA) for every lot is to be had for download.

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All plenty of Myocyte Growth Media are subjected to complete best manipulate trying out. These checks take a look at for increase-selling activity, adherence rate, and anticipated mobileular morphology the use of cultured number one human cardiac myocytes. Approved in-residence plenty of media are used as a reference. Additionally, microbial trying out guarantees that every one plenty of Myocyte Growth Media are freed from microbial contaminants (fungi, bacteria, mycoplasma).

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