AE-1465 EzFastBlot | 2332590

ATTO Western Blotting Semi Dry Blotting System WSE-4115 WSE-4020 WSE-4040 Built-in Power Supply unit PoweredBlot-Ace For 2 mini gels HorizeBLOT 2M-R For four mini gels HorizeBLOT 4M-R WSE-4115 WSE-4020 WSE-4040 2 ATTO Western Blotting System

INTRODUCTION

What’s Semi Dry Blotting? Western blotting is the simple experimental technique to in particular and excessive-sensitively hit upon favored aspect from protein sample. It is more or less divided into 2 types;”Semi Dry kind” and “Wet kind (Tank kind)”.

We, ATTO, advise and offer Semi Dry kind blotting gadget primarily based totally on the subsequent merits. Merit 1 Less Amount of Blotting Solution Semi dry kind is the technique requiring small quantity of blotting answer wherein immerses clear out out. (Wet kind wishes extra blotting answer for immersing entire of clear out out, gel, membrane and electrode.) Related Products Specification HorizeBLOT 2M-R/4M-R PoweredBlot-Ace Merit 2 Excellent Operability The step of semi dry kind;stacking clear out out, gel and membrane horizontally among electrodes earlier than blotting is easier than that of moist kind.  Merit three Merit four Blotting answer for semi dry kind Short Operating Time Blotting answer for moist kind Merit five More Effective With Optimized Blotting Solution AE-1460 EzBlot stacking order AE-1465 EzFastBlot stacking order Small Heating Value In the case of semi dry kind, heating cost is small as it calls for small cutting-edge for blotting.

Operation of semi dry kind blotting is finished in a quick time

  •  ATTO advise AE-1460 EzBlot for most effective semi dry blotting whose aspect is made from three forms of answer and AE-1465 EzFastBlot for excessive velocity blotting whose aspect is made from 1 form of answer.
  • Transfer performance is impoved via way of means of the use of optimized blotting answer* withinside the case of semi dry kind. * Kyhse-Andersen J et al., (1984)
  • Electroblotting of a couple of gels: a easy equipment with out buffer tank for speedy switch of proteins from polyacrylamide to nitrocellulose.
  • J Biochem Biophys Methods.10: Blotting answer along with 25mM Tris, 192mM Glycine, 20% Methanol isn’t always endorsed to apply for semi dry kind.
  • Transfer performance is extensively deteriorated due to the fact those answer aren’t optimized for semi dry kind. Cathode (-) Anode(+) Cathode (-) Anode (+) EzBlot C answer:clear out out three EzBlot B answer:gel EzBlot B answer:membrane EzBlot B answer:clear out out EzBlot
  • A Solution:Filter 2 EzFastBlot:Filter 2 EzFastBlot:Gel EzFastBlot:Membrane EzFastBlot:Filter 2 01 The distinction of switch performance of every buffer Tris-Glycine-MeOH answer AE-1460 EzBlot These photographs display that AE-1460 EzBlot is extra powerful in semi dry kind blotting.

Buit-in Power Supply Unit Type

Blotting System WSE-4115 PoweredBlot-Ace Simple opearation simply with choosing mode Built-in electricity deliver unit, Save area 2 selectable mode ;”Standard mode” and “Rapid mode” STD (Standard) mode(steady voltage 12V equal to steady cutting-edge 2mA/cm 2 ) Blotting time:30-60 min Timer:Selectable variety is 1-200min.

RAPID mode(steady voltage 24V equal to contant cutting-edge 7-8mA/cm 2 ) Blotting time:10-30min Timer:Selectable variety is 1-200min. Required area for placing primary unit; A5 length (148mm 210mm) spring shape withinside the top electrode unit which permit to push the stack with appropriate strain How to apply 1 Remove lid as electricity deliver unit.

Product aspect 2 Separate the decrease electrode from the top, and stack clear out out, gel and membrane at the decrease. Product code Model Name WSE-4115 PoweredBlot-Ace three Use connected curler for getting rid of air bubbles WSE-4115 C PoweredBlot-Ace (With Compact length P+ membrane & Filter) WSE-4115 M PoweredBlot-Ace (With Mini length P+ membrane & Filter) ATTO Western Blotting System Electrode length 98mm 95mm Example of gel/ membrane placing Compact gel x1 Mini gel x1 High velocity mode with out electricity deliver device 83mm 90mm four Select a method and press RUN button.The operation starts.

PoweredBlot-Ace HorizeBLOT 2M-R/4M-R Specification Related Products

024 ATTO Western Blotting System INTRODUCTION Semi Dry Blotting System WSE-4020 HorizeBLOT 2M-R (For 2 Mini gel) 100mm Electrode length 205mm Example of gel/ membrane placing Related Products Specification HorizeBLOT 2M-R/4M-R PoweredBlot-Ace High velocity blotting Improved operability and reproducibility Semi Dry Blotting System WSE-4040 HorizeBLOT 4M-R High velocity blotting Suitable for Large gel and membrane (For four Mini gel) Compact gel x2 Compact gel x3 Mini gel x2 200mm Electrode length 205mm Example of gel/ membrane placing Compact gel x4 (Max nine gels) Mini gel x4 Slab gel x1 83mm 83mm 90mm 90mm 130mm 138mm 03 five ATTO

Western Blotting System Spring shape withinside the top electrode unit which permit to push the stack with appropriate strain Easy placing with aligning a part of primary unit for adjusting of the quilt and base Latch technique for solving the top and decrease electrode; stopping clear out out, membrane and gel from slipping out of function Reverse connection prevention shape of lead cord How to apply 1 Remove the quilt (top electrode) from the base (decrease electrode). 2 Stack clear out out, membrane and gel at the decrease electorde, and do away with air bubbles with curler.

Product aspect Comparative records

Blotting answer Blotting tool Transfer time 200kDa 40kDa ATTO W.B. System AE-1465 EzFastBlot HorizeBLOT 2M-R High velocity blotting C.V. 25V, 7min. Company L W.B. System High velocity blotting package Semi dry kind blotting gadget for excessive velocity blotting High velocity blotting 7min. This records indicates that switch performance of ATTO Western Blotting System is better than that of the comparion in excessive polymeric three Set the top electrode at the decrease and join electricity deliver device with lead cord. Product code Model Name WSE-4020 HorizeBLOT 2M-R WSE-4020 M HorizeBLOT 2M-R(With Mini slab length P+ membrane & Filter) WSE-4040 HorizeBLOT 4M-R WSE-4040 M HorizeBLOT 4M-R(With Mini slab length P+ membrane & Filter) Roller for blotting Power lead cord for HorizeBLOT-R 200kDa 40kDa 04 Tips Roller for blotting four Press RUN button of the electricity deliver device and begin blotting. Nonuniformity via way of means of air bubbles Roller Unused Roller Used Air bubbles among clear out out, membrane and gel might also additionally motive troubles along with nonuniformity. Using curler for blotting can do away with effortlessly air bubbles.

AE-1465 EzFastBlot

2332590 Atto 3unit 73.7 EUR

WSE-7210 EzFastBlot HMW

2332595 Atto 3unit 64.86 EUR

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The following merchandise encompass curler standardly. WSE-4115 PoweredBlot-Ace WSE-4020 HorizeBLOT 2M-R WSE-4040 HorizeBLOT 4M-R INTRODUCTION PoweredBlot-Ace HorizeBLOT 2M-R/4M-R Specificatio Related Products

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