Lipid Peroxidation (MDA + HNE) Assay Bundle

Lipid Peroxidation (MDA) Assay Bundle (Colorimetric/Fluorometric) (ab118970) provides a helpful software program for delicate detection of malondialdehyde (MDA).

Inside the lipid peroxidation assay protocol, the MDA inside the sample reacts with thiobarbituric acid (TBA) to generate a MDA-TBA adduct. The MDA-TBA adduct might be merely quantified colorimetrically (OD = 532 nm) or fluorometrically (Ex/Em = 532/553 nm). This assay detects MDA ranges as little as 1 nmol/successfully colorimetrically and 0.1 nmol/successfully fluorometrically.

The MDA assay can be refered to as a TBARS assay.

Chinese language language protocol on the market. See protocols half beneath.

For an alternate MDA assay, with out the heating steps required inside the TBARS assay, try MDA assay ab233471.

Notes

Lipid peroxidation refers again to the oxidative degradation of lipids. On this course of free radicals take electrons from the lipids (usually in cell membranes), resulting in cell hurt. Quantification of lipid peroxidation is essential to judge oxidative stress. Lipid peroxidation varieties reactive aldehydes similar to malondialdehyde (MDA) and 4-hydroxynonenal (4- HNE) as pure bi-products. Malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE) are typically used as markers of lipid peroxidation, and to assay for oxidative hurt / oxidative stress.

Related merchandise

Evaluation the oxidative stress marker and assay info, or the whole metabolism assay info to check additional assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and as well as how one can assay metabolic function in dwell cells using your plate reader.

Moreover see the favored 4-HNE Assay Bundle ab238538 instead marker of lipid peroxidation and oxidative stress.

How totally different researchers have used Lipid Peroxidation Assay Bundle ab118970

The MDA/TBARs assay gear has been utilized in publications in a variety of sample types, along with:
– Human: serum¹, hippocampal principal cell extracts², A375 cultured cell lysates³, plasma and platelet samples⁴
– Mouse: neuronal cell lysates⁵, coronary coronary heart tissue extract⁶, plasma⁷, cell extracts⁸
– Rat: hippocampal tissue extracts⁹, cardiomyocyte extracts of cultured cells¹⁰, lung lysates¹¹
– Pig: serum¹²

^{References: 1 – Shen J et al. 2018, 2 – Wang Q et al. 2019, 3 – Luo M et al. 2018,  4 – Mustafa AG et al. 2018, 5 – Murphy Okay et al. 2018, 6 – Guan F et al. 2019, 7 – Costa CRC et al. 2018, 8 – Eleftheriadis T et al. 2019, 9 – Malekiyan et al. 2019, 10 – Zhou Z et al. 2018, 11 – Li L et al. 2018, 12 – Lee SE and Kang KS 2019}